Lipase Activity in the Murine Acquired Immune Deficiency Syndrome (MAIDS) model

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dc.contributorNunez, Meganen_US
dc.contributorFrary, Amyen_US
dc.contributor.advisorStranford, Sharonen_US
dc.contributor.authorHerman, Nicoleen_US
dc.date.accessioned2011-02-16T13:46:44Z
dc.date.available2011-02-16T13:46:44Z
dc.date.gradyear2010en_US
dc.date.issued2011-02-16
dc.date.submitted2010-05-13 14:22:03en_US
dc.description.abstractMurine acquired immune deficiency syndrome (MAIDS) is a retrovirally induced disease caused by exposure to the murine leukemia virus (MuLV) and is used to study human AIDS. Two strains of mice are used in the model system; the BALB/c mice, which are resistant to the progression of MAIDS and the C57BL/6 mice, which are susceptible and progress to MAIDS with time. A DNA microarray analysis was used to recognize differentially expressed genes in lymph nodes and spleen at three and seven days post infection in both strains. Pancreatic lipase was found to be differentially expressed over 100 fold in the spleen at three days post infection and is thought to be resistance-associated. To investigate the activity of pancreatic lipase, a colorimetric assay was used to compare spleen lysates from naïve, three day and seven day infected mice. Results suggest higher levels of activity in the naïve and infected BALB/c mice compared to C57BL/6 animals. Large biological variability in the lipase activity within each condition suggests that the trend is not statistically significant. Therefore, it is unlikely that pancreatic lipase is playing an essential role in the recovery of the BALB/c mice.en_US
dc.description.sponsorshipBiological Sciencesen_US
dc.description.sponsorshipSpanishen_US
dc.identifier.urihttp://hdl.handle.net/10166/623
dc.language.isoen_USen_US
dc.rights.restrictedpublic
dc.titleLipase Activity in the Murine Acquired Immune Deficiency Syndrome (MAIDS) modelen_US
dc.typeThesisen_US
mhc.degreeUndergraduateen_US
mhc.institutionMount Holyoke Collegeen_US

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