Lipase Activity in the Murine Acquired Immune Deficiency Syndrome (MAIDS) model



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Murine acquired immune deficiency syndrome (MAIDS) is a retrovirally induced disease caused by exposure to the murine leukemia virus (MuLV) and is used to study human AIDS. Two strains of mice are used in the model system; the BALB/c mice, which are resistant to the progression of MAIDS and the C57BL/6 mice, which are susceptible and progress to MAIDS with time. A DNA microarray analysis was used to recognize differentially expressed genes in lymph nodes and spleen at three and seven days post infection in both strains. Pancreatic lipase was found to be differentially expressed over 100 fold in the spleen at three days post infection and is thought to be resistance-associated. To investigate the activity of pancreatic lipase, a colorimetric assay was used to compare spleen lysates from naïve, three day and seven day infected mice. Results suggest higher levels of activity in the naïve and infected BALB/c mice compared to C57BL/6 animals. Large biological variability in the lipase activity within each condition suggests that the trend is not statistically significant. Therefore, it is unlikely that pancreatic lipase is playing an essential role in the recovery of the BALB/c mice.