Differential Expression of an Endogenous Retrovirus in MAIDS Susceptible (C57BL/6) Versus Resistant (BALB/c) Mice
| dc.contributor | Bacon, Sarah | en_US |
| dc.contributor | Peterson, Mark | en_US |
| dc.contributor.advisor | Stranford, Sharon | en_US |
| dc.contributor.author | Socha, Amanda | en_US |
| dc.date.accessioned | 2011-02-16T13:47:25Z | |
| dc.date.available | 2011-02-16T13:47:25Z | |
| dc.date.gradyear | 2006 | en_US |
| dc.date.issued | 2011-02-16 | |
| dc.date.submitted | 2006-05-26 14:31:32 | en_US |
| dc.description.abstract | Murine acquired immunodeficiency syndrome (MAIDS) is a retrovirus-induced disease in mice that results from infection with a specific mixture of Murine Leukemia Virus (MuLV) known as LP-BM5. The two strains of mice used in this research are either the disease susceptible strain (C57BL/6 or BL/6) or the disease resistant strain (BALB/c). The Tepsuporn thesis in the Stranford lab at Mount Holyoke College generated differential gene expression data from a DNA-microarray based assay of MAIDS susceptible versus resistant mice that were either injected with virus or mock supernatant. One gene in particular, mink cell focus-forming virus (MCF), had a 55-fold higher expression in disease susceptible mice when compared to resistant mice under any conditions. MCF is a component of LP-BM5 MuLV and was originally used to track virus expression in host cells. The objective of this research is to utilize Reverse-Transcriptase PCR and Real-Time RT-PCR to determine whether or not MCF is found in the genome of mice and expressed as mRNA. Data shows that both BL/6 and BALB/c mice, when naïve from injection with virus or mock supernatant, expressed MCF mRNA in both the spleen and the lymph node and contained MCF in their genomes. Real time RT-PCR data analysis suggests that naïve mice express higher levels of MCF-like mRNA in the BL/6 mice when compared to the BALB/c mice; this information contradicts the microarray data. It is possible that prior to infection with virus or mock supernatant, MCF-like sequences are suppressed in BALB/c mice and upregulated in BL/6 mice. Future studies of MCF and its implications disease pathogenesis may lead to a better understanding of the immune response to HIV and discover new factors that contribute to human susceptibility to AIDS. | en_US |
| dc.description.sponsorship | Biological Sciences | en_US |
| dc.identifier.uri | http://hdl.handle.net/10166/757 | |
| dc.language.iso | en_US | en_US |
| dc.rights.restricted | public | |
| dc.title | Differential Expression of an Endogenous Retrovirus in MAIDS Susceptible (C57BL/6) Versus Resistant (BALB/c) Mice | en_US |
| dc.type | Thesis | en_US |
| mhc.degree | Undergraduate | en_US |
| mhc.institution | Mount Holyoke College | en_US |
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