Testing the occurrence of forward hyper-translocation during the promoter escape transition

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dc.contributor.advisorHsu, Lilian
dc.contributor.authorJiang, Yunnan
dc.date.accessioned2013-05-01T17:24:24Z
dc.date.available2013-05-01T17:24:24Z
dc.date.gradyear2009en_US
dc.date.issued2013-05-01
dc.description.abstractThe macromolecular process of RNA transcription is composed of three phases, initiation, elongation and termination. At many promoters, RNA polymerase (RNAP) repetitively aborts short RNAs 2 to 15 nucleotides (nts) in length before successfully making the transition to the elongation phase. Mutations in the initial transcribed sequence (ITS) region, from +3 to +10, of Eσ70-dependent T5 N25 promoter lengthen the abortive initiation program, and lead to the formation of the very long abortive transcripts (VLATs) of 16 to 21 nts. Recent published work done in the lab suggests that the VLATs formation may result from forward hyper-translocation movement of RNAP during the escape transition. This project is aimed to test the physical occurrence of forward hyper-transcription by binding a non-cleaving E111Q EcoRI protein roadblock to stall the forward translocating RNAP and thus prevent the formation of VLATs.en_US
dc.description.sponsorshipBiochemistryen_US
dc.identifier.urihttp://hdl.handle.net/10166/3211
dc.language.isoen_USen_US
dc.rights.restrictedpublicen_US
dc.subjectEscherichia colien_US
dc.subjectRNA polymerasesen_US
dc.subjectGenetic transcriptionen_US
dc.titleTesting the occurrence of forward hyper-translocation during the promoter escape transitionen_US
dc.typeThesis
mhc.degreeUndergraduateen_US
mhc.institutionMount Holyoke College

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