Motors and Mitosis: Characterizing the Role of Dynein in Mammalian Epithelial Spindle Positioning Phenomena

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dc.contributorWadsworth, Paten_US
dc.contributorPeterson, Marken_US
dc.contributorStranford, Sharonen_US
dc.contributor.advisorFink, Rachelen_US
dc.contributor.authorBrooke, Kaitlinen_US
dc.date.accessioned2011-02-16T13:47:22Z
dc.date.available2011-02-16T13:47:22Z
dc.date.gradyear2010en_US
dc.date.issued2011-02-16
dc.date.submitted2010-05-16 11:55:35en_US
dc.description.abstractReproduction distinguishes living organisms from inanimate objects, as cells cannot be made except by division from preexisting cells. Despite the importance of proper cell division for growth, aging, healing, and the prevention of diseases such as cancer, many of the complicated protein interactions involved remain a mystery. Mitosis, the segregation of replicated chromosomes to two new daughter cells, is central to the process of cell division. The forces generated by motor proteins, a special class of proteins able to convert chemical energy from ATP into mechanical energy, are necessary for the movements of the cytoskeleton during mitosis. Dynein is a motor protein that walks along the microtubules of the mitotic spindle, performing several essential roles throughout the phases of mitosis. Research in budding yeast and other model systems has implicated dynein located at the cell cortex as a key player in spindle positioning (Kozlowski et al., 2007; Carminati and Stearns, 1997). The regulation and mechanics of spindle positioning are poorly understood yet critical for both asymmetric and symmetric cell divisions. The characterization of spindle positioning phenomena in a line of mammalian epithelial cells revealed a pattern of asymmetric spindle position at anaphase onset followed by unequal movement of each set of sister chromatids in a significant subpopulation of cells. In addition to phase contrast observation of spindle positioning, microscopic observation of mammalian epithelial cells expressing fluorescent gamma tubulin revealed asymmetric movement of each spindle pole during anaphase. Immunofluorescent fixing and staining of mammalian epithelial cells suggested that dynein localized to the cell cortex plays a role in spindle positioning phenomena.en_US
dc.description.sponsorshipBiological Sciencesen_US
dc.identifier.urihttp://hdl.handle.net/10166/741
dc.language.isoen_USen_US
dc.rights.restrictedpublic
dc.subjectCell divisionen_US
dc.subjectMitosisen_US
dc.subjectMotor proteinsen_US
dc.titleMotors and Mitosis: Characterizing the Role of Dynein in Mammalian Epithelial Spindle Positioning Phenomenaen_US
dc.typeThesisen_US
mhc.degreeUndergraduateen_US
mhc.institutionMount Holyoke Collegeen_US

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