Preparation and Surface PEGylation of Crosslinked Polydimethylsiloxane Substrates with Tunable Moduli

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dc.contributorGomez, Maria
dc.contributorAidala, Katherine
dc.contributor.advisorChen, Wei
dc.contributor.authorWang, Wanxin
dc.date.accessioned2013-05-14T12:53:09Z
dc.date.available2013-05-14T12:53:09Z
dc.date.gradyear2013en_US
dc.date.issued2013-05-14
dc.description.abstractMechanical patterns on cell culture substrates can be potentially used as effective cues in directing cell migrations. Fully functional substrates require not only readily tunable modulus to generate mechanical stimuli but also proper surface properties to ensure proper interactions with the surrounding media. In this study, crosslinked polydimethylsiloxane (PDMS) with tunable modulus ranging from 50 to 1000 kPa was fabricated using platinum-catalyzed hydrosilylation between vinyl and -SiH functionalized PDMS pre-polymers. Substrate modulus was tuned as a function of the density of the reactive moieties (-SiH) on crosslinkers, the molar ratio between vinyl and -SiH functionalities, and the average molecular weight between adjacent crosslinkers. To overcome the hydrophobicity of PDMS surfaces, mono-vinyl-terminated polyethylene glycol (V-PEG) was grafted onto crosslinked PDMS thin films containing excess -SiH functionality via surface hydrosilylation reaction. Efficiency of surface PEGylation was controlled by surface density of -SiH groups, molecular weight of V-PEG, and catalyst concentrations.en_US
dc.description.sponsorshipChemistryen_US
dc.identifier.urihttp://hdl.handle.net/10166/3232
dc.language.isoen_USen_US
dc.rights.restrictedpublicen_US
dc.subjectPolydimethylsiloxaneen_US
dc.subjectCrosslinkeden_US
dc.subjectHydrosilylationen_US
dc.subjectYoung's modulusen_US
dc.subjectPEGylationen_US
dc.subjectMechanotaxisen_US
dc.titlePreparation and Surface PEGylation of Crosslinked Polydimethylsiloxane Substrates with Tunable Modulien_US
dc.typeThesis
mhc.degreeUndergraduateen_US
mhc.institutionMount Holyoke College

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