Mouse Liver Development: Analyzing the Relationship Between the Liver and Endothelial Cells Through Whole Mount In Situ Hybridization

Simple item page
dc.contributorBacon, Sarah
dc.contributorTremblay, Kimberly
dc.contributor.advisorFink, Rachel
dc.contributor.authorClark, Emily
dc.date.accessioned2018-05-09T15:10:27Z
dc.date.available2018-05-09T15:10:27Z
dc.date.gradyear2018en_US
dc.date.issued2018-05-09
dc.description.abstractThe liver’s function as a filtering organ leaves it vulnerable to toxin- related diseases such as cirrhosis, and cancer. Studying liver development in the murine embryo allows for in vivo investigation of the process in a mammalian system. Insight from this study may lead to development of novel therapies to treat liver disease. Fate mapping has demonstrated that the murine liver begins as two progenitor populations in the definitive endoderm, when the embryo has 4-6 somites. These precursor cells contribute differently to the liver, and RNA sequencing has identified genes that are differentially expressed between these populations. We sought to further confirm the transcriptional differences between the two populations and probe later expression during development using whole mount in situ hybridization (WISH). This technique allows for reliable visualization of tissues expressing the gene of interest. Although these genes were initially identified as endoderm specific, many were present in other tissues. Interestingly, 20% showed apparent endothelial expression throughout early development.  Of these genes, few showed endothelial cell expression in major vessels; instead expression was more likely to be found in vessels proximal to gut organs. These results suggest a more complex relationship between the endoderm and endothelial cells than previously assumed.en_US
dc.description.sponsorshipBiological Sciencesen_US
dc.identifier.urihttp://hdl.handle.net/10166/4632
dc.language.isoen_USen_US
dc.rights.restrictedrestricteden_US
dc.subjectLiveren_US
dc.subjectdevelopmenten_US
dc.subjectmouseen_US
dc.subjectmurineen_US
dc.subjectwhole mount in situ hybridizationen_US
dc.subjectin situ hybridizationen_US
dc.subjectendothelial cellsen_US
dc.subjecthepatoblastsen_US
dc.titleMouse Liver Development: Analyzing the Relationship Between the Liver and Endothelial Cells Through Whole Mount In Situ Hybridizationen_US
dc.typeThesis
mhc.degreeUndergraduateen_US
mhc.institutionMount Holyoke College

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Emily Clark Thesis Final Draft.pdf
Size:
13.8 MB
Format:
Adobe Portable Document Format
Download

Collections

Student Theses and Honors Collection