Coordination between Insulin Signaling and Ecdysone Signaling during Drosophila melanogaster Development

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dc.contributorJayathilake, Himali
dc.contributorKnight, Jeff
dc.contributor.advisorWoodard, Craig
dc.contributor.authorWoldemeskel, Bezawit
dc.date.accessioned2014-06-25T13:20:00Z
dc.date.available2014-06-25T13:20:00Z
dc.date.gradyear2014en_US
dc.date.issued2014-06-25
dc.description.abstractInsulin signaling is a remarkably conserved pathway that regulates nutrient storage and release in vertebrates and invertebrates. In Drosophila, growth and development are balanced through a mechanism involving insulin and ecdysone signaling. The level of insulin signaling varies based on the stage of development to regulate the release and storage of nutrients in the fat body, an organ that serves as a nutrient reservoir. During metamorphosis, insulin signaling is downregulated to release nutrients from the fat body necessary to fuel the process, while at the same time, a pulse of ecdysone signaling remodels the fat body from a sheet of attached polygonal cells to individual cells that are dispersed through out the body cavity. Previous studies have indicated that ecdysone signaling negatively regulates insulin signaling expression during metamorphosis. I proposed that ecdysone signaling coordinates the break up and dispersal of fat body cells with nutrient release from the fat body by downregulating insulin signaling. I tested this hypothesis by examining markers of insulin signaling in mosaic clones of fat body cells in which ecdysone signaling is disrupted. My results indicate that ecdysone is necessary to inhibit insulin signaling during fat body remodeling.en_US
dc.description.sponsorshipBiochemistryen_US
dc.identifier.urihttp://hdl.handle.net/10166/3530
dc.language.isoen_USen_US
dc.rights.restrictedrestricteden_US
dc.subjectInsulin signalingen_US
dc.subjectFat body remodelingen_US
dc.subjectDrosophila melanogasteren_US
dc.subjectautophagyen_US
dc.subjectEcdysone signalingen_US
dc.titleCoordination between Insulin Signaling and Ecdysone Signaling during Drosophila melanogaster Developmenten_US
dc.typeThesis
mhc.degreeUndergraduateen_US
mhc.institutionMount Holyoke College

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