• Login
    View Item 
    •   IDA Home
    • Students -- Research, Data, Projects, and Papers
    • Student Theses and Honors Collection
    • View Item
    •   IDA Home
    • Students -- Research, Data, Projects, and Papers
    • Student Theses and Honors Collection
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    The role of Aur-A in Drosophila melanogaster fat body remodeling

    Thumbnail
    View/Open
    D.A-Final Thesis.pdf (38.43Mb)
    Date
    2017-06-29
    Author
    Arshinoff, Danielle
    Metadata
    Show full item record
    Abstract
    Tissue remodeling is an important process in multicellular organisms that is necessary for processes such as wound healing and tumor metastasis. For remodeling to occur, connective proteins of the extracellular matrix (ECM) must be cleaved so that cells can migrate. The migration of cells in wound healing promotes wound closure and ECM degradation allows cancerous cells to separate from the tumor to metastasize. Drosophila melanogaster undergoes metamorphosis from larva to adult. Most larval tissues undergo apoptosis, but the larval fat body undergoes remodeling and persists until after the adult has eclosed. During remodeling, the larval fat body cells change from being polygonal cells connected in sheets to spherical cells that are free floating. Since D. melanogaster possess multiple tools for genetic analysis, this organism is a commonly used model organism in biomedical research. Drosophila were fed EMS to establish mutations on their third chromosome and were then scored for lethality. Twenty of these lines are pharate adult lethal, wherein the newly formed adult flies fail to eclose from their pupal case, and have larval fat body remodeling abnormalities. The fat body phenotype was screened and categorized according to the level of remodeling. The chromosomal location of four of the mutated genes that cause pharate adult lethality and abnormal fat body remodeling was attempted to be established. Three of the mutated lines were determined to have mutations in the aur-A gene. This gene is known to affect the cytoskeleton arrangement in neuroblasts, but its role in fat body remodeling is unknown. The results from this study suggest that that this mutated gene does affect the actin filament organization.
    URI
    http://hdl.handle.net/10166/4059
    Collections
    • Student Theses and Honors Collection

    DSpace software copyright © 2002-2015  DuraSpace
    Contact Us | Send Feedback | MHC Accessibility Barriers Form
    Theme by 
    @mire NV
     

     

    Browse

    All of IDACommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2015  DuraSpace
    Contact Us | Send Feedback | MHC Accessibility Barriers Form
    Theme by 
    @mire NV