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Crosslinking the 3′ End of a VLAT to RNA Polymerase: A Method to Elucidate Forward Hyper-Translocation
(2012-05-09)
On a typically strong E. coli promoter, as nascent RNA grows to 8-10 nt⎯accompanied by scrunching 6-8 bp of the enlarged transcription bubble⎯the initial DNA-RNAP contacts disrupt, upstream bubble rewinds, promoter escape ...