Show simple item record

dc.contributorPeterson, Mark
dc.contributorHsu, Lilian
dc.contributor.advisorWoodard, Craig
dc.contributor.authorCossogue, Anne Sarie Y.
dc.date.accessioned2012-05-29T13:06:20Z
dc.date.available2012-05-29T13:06:20Z
dc.date.issued2012-05-29
dc.identifier.urihttp://hdl.handle.net/10166/1044
dc.description.abstractDuring metamorphosis in Drosophila melanogaster some larval tissues are destroyed through programmed cell death (PCD) (Aguila et al., 2007). The steroid hormone 20-hydroxyecdysone (ecdysone) regulates several genes that induce PCD in Drosophila (McBrayer et al 2007). Apoptosis, which is a type of PCD, occurs when the antiapoptotic gene diap1 is downregulated in larval tissues. DIAP1 regulates apoptosis by deactivating caspases (Orme and Meier, 2009). During metamorphosis, the larval fat body, which is composed of a sheet of connected cells, is not destroyed by PCD. This organ is instead remodeled and is transformed into individual, spherical, and loose cells (Nelliot et al., 2006). I hypothesize that diap1 is expressed in the larval fat body throughout metamorphosis and that this helps to inhibit PCD in this organ. To test this hypothesis, I examined diap1 transcript levels in wild type Drosophila using qPCR. In addition, I determined the expression of diap1 in larval fat body of flies with a diap1 RNAi construct. My results demonstrate that diap1 is expressed in the larval fat body during prepupal and early pupal development. Preliminary findings showed that Drosophila expressing the diap1 RNAi construct failed to undergo normal fat body remodeling. This finding suggests that diap1 is necessary for the normal timing of fat body remodeling and the successful development of Drosophila.en_US
dc.description.sponsorshipBiological Sciencesen_US
dc.language.isoen_USen_US
dc.subjectApoptosisen_US
dc.subjectFat body remodelingen_US
dc.subjectProgrammed cell deathen_US
dc.subjectdiap1en_US
dc.subjectAutophagyen_US
dc.subject20-hydroxyecdysoneen_US
dc.titleTHE ROLE OF DROSOPHILA INHIBITOR OF APOPTOSIS (DIAP1) IN FAT BODY REMODELINGen_US
dc.typeThesis
dc.date.gradyear2012en_US
mhc.institutionMount Holyoke College
mhc.degreeUndergraduateen_US
dc.rights.restrictedpublic


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record